A New In-sight for Quantitative Biochemistry: Studies on Thermodynamic and Kinetic Aspects of Enzyme-catalyzed Reaction using Papain and Bromelain as Model Enzymes

Thermodynamic data on enzyme-catalyzed reactions play an essential role in the prediction of the extent of reaction and the position of equilibrium for any process in which these reactions occur. The importance of this information lies in its applications both in biochemistry and in industrial enzymology. The data are also essential for metabolic control analysis (MCA): an important part of systemic biology. Specifically, the full application of MCA requires both thermodynamic and kinetic data as well as knowledge of metabolite concentrations. The result is a quantitative description of metabolic processes, a detailed understanding of which may lead to an understanding of the differences between the normal and the diseased states of the living system as well as the ability to successfully engineer organisms to accomplish specific aims. It is, therefore, aimed at studying on kinetic and thermodynamic aspects of enzyme catalyzed reactions using Papain and Bromelain as model enzymes.